Characterization of the Leptospiral Outer Membrane and Description of Three Novel Leptospiral Membrane Proteins
نویسندگان
چکیده
منابع مشابه
Characterization of the leptospiral outer membrane and description of three novel leptospiral membrane proteins.
The outer membrane (OM) of the mammalian pathogen Leptospira kirschneri was isolated in the form of membrane vesicles by alkaline plasmolysis and separated from the protoplasmic cylinder by sucrose density gradient ultracentrifugation. All four components of the alkaline plasmolysis buffer, including 1.0 M NaCl, 27% sucrose (wt/vol), 2 mM EDTA, and 10 mM Tris (pH 9), were required for efficient...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 2002
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.70.9.4936-4945.2002